neck cancer cell lines 22rv1  (ATCC)

Journal: Cell Death & Disease

Article Title: Genomic and transcriptomic profiling of radioresistant prostate and head and neck cancers implicate a BAHD1-dependent modification of DNA damage at the heterochromatin

doi: 10.1038/s41419-024-07316-y

Figure Lengend Snippet: A Differentially expressed genes (DEGs) in 22Rv1 and DU145, and ( B ) in FaDu and DU145. The DEGs were defined if the adjusted P < 0.05. C Expression profile of 221 common DEGs with consistent dysregulation direction in both 22Rv1 and DU145. D Expression profile of 1420 common DEGs with consistent dysregulation direction in both FaDu and HK1. Samples were ordered by cell lines and phenotype in both figures. E 18 DEGs with consistent dysregulation direction across four cell lines. The colour of each dot indicates the dysregulation direction, with red indicating upregulation and blue indicating downregulation. The size of each dot varies based on its fold change. The intensity of colour within each box reflects the range of P -values. F Significant pathways involved by the 5 upregulated DEGs across the 4 cell lines, curated from over-representation analysis (false discovery rate <0.1). The colour intensity within each colour filled box represents the rich factor of the gene in the pathway.

Article Snippet: Prostate and head and neck cancer cell lines 22Rv1, DU145, and FaDu were purchased from the American Type Culture Collection (ATCC), routinely tested for mycoplasma contamination with EZ-PCT TM Mycoplasma Detection Kit (Biological Industries, USA) and authenticated using short tandem repeat analysis by ATCC (ATCC, USA).

Techniques: Expressing

Journal: Cell Death & Disease

Article Title: Genomic and transcriptomic profiling of radioresistant prostate and head and neck cancers implicate a BAHD1-dependent modification of DNA damage at the heterochromatin

doi: 10.1038/s41419-024-07316-y

Figure Lengend Snippet: A The representative western blot showed the changes in the expression of DSB, DNA repair, cell cycle arrest and heterochromatin markers under different time points post-4 Gy IR (normalised against control), GAPDH was used as a loading control. B and C-top panel Representative images of H3K9me3 (green) and H3K27me3 (red) foci in 22Rv1 cells at 0 and 1 h treatment time points and FaDu cells at 0 and 6 h treatment time points. Scale bar: 5 μm. B and C-bottom panel H3K9me3 intensity was quantified in AUCs and represented as percentage frequencies, compared between RR and WT; H3K27me3 foci were quantified as mean foci per cell, bars represent mean±SD, n = 3 per group. DSB DNA double-strand break, AUC area under the curve, WT wild-type, SD standard deviation.

Article Snippet: Prostate and head and neck cancer cell lines 22Rv1, DU145, and FaDu were purchased from the American Type Culture Collection (ATCC), routinely tested for mycoplasma contamination with EZ-PCT TM Mycoplasma Detection Kit (Biological Industries, USA) and authenticated using short tandem repeat analysis by ATCC (ATCC, USA).

Techniques: Western Blot, Expressing, Control, Standard Deviation

Journal: Cell Death & Disease

Article Title: Genomic and transcriptomic profiling of radioresistant prostate and head and neck cancers implicate a BAHD1-dependent modification of DNA damage at the heterochromatin

doi: 10.1038/s41419-024-07316-y

Figure Lengend Snippet: A and B-top left Representative images of H3K9me3 (green) and H3K27me3 (red) foci in 22Rv1 cells at 1 h and FaDu cells at 6 h post-siBAHD1 treatment. Scale bar: 5 μm; (top right) Representative western blot showed the changes in H3K9me3 and H3K27me3 protein levels in 22Rv1 cells at 1 h and FaDu cells at 6 h post-siBAHD1 treatment; (bottom panel) H3K9me3 intensity was quantified in AUCs and represented as percentage frequencies, compared between RR and WT; H3K27me3 foci were quantified as mean foci per cell, bars represent mean±SD, n = 3 per group. C Colony forming assay of 22Rv1 and FaDu cells under different IR dosages, with and without siBAHD1 treatment, n = 3 per group. Asterisk indicates significance between siBAHD1-treated and control groups. Asterisk indicates P < 0.05.

Article Snippet: Prostate and head and neck cancer cell lines 22Rv1, DU145, and FaDu were purchased from the American Type Culture Collection (ATCC), routinely tested for mycoplasma contamination with EZ-PCT TM Mycoplasma Detection Kit (Biological Industries, USA) and authenticated using short tandem repeat analysis by ATCC (ATCC, USA).

Techniques: Western Blot, Control

Journal: Cell Death & Disease

Article Title: Genomic and transcriptomic profiling of radioresistant prostate and head and neck cancers implicate a BAHD1-dependent modification of DNA damage at the heterochromatin

doi: 10.1038/s41419-024-07316-y

Figure Lengend Snippet: A A hypothesised model showing BAHD1 contributes to the enhanced heterochromatin response in RR cancer cells, whereby increased repair efficiency following irradiation (IR)-induced damage leads to decreased radiosensitivity. The inhibition of BAHD1 leads to chromatin unpacking, decreased repair efficiency and improved radiosensitivity (created with Biorender.com). B and C, left Representative images of co-localised γH2AX (green) and p-53BP1 (red) foci in 22Rv1-RR and FaDu-RR cells at 1 and 6 h post-IR, with and without siBAHD1 treatment. Scale bar: 5 μm; ( right ) Quantification of co-localised γH2AX and p-53BP1 mean foci per cell, bars represent mean±SD, n = 3 per group. D, E Representative western blot showed the changes in the expression of DSB, DNA repair, cell cycle arrest and heterochromatin markers of 22Rv1-RR cells and FaDu-RR cells at 1 and 6 h post-IR, with and without siBAHD1 treatment (normalised against control).

Article Snippet: Prostate and head and neck cancer cell lines 22Rv1, DU145, and FaDu were purchased from the American Type Culture Collection (ATCC), routinely tested for mycoplasma contamination with EZ-PCT TM Mycoplasma Detection Kit (Biological Industries, USA) and authenticated using short tandem repeat analysis by ATCC (ATCC, USA).

Techniques: Irradiation, Inhibition, Western Blot, Expressing, Control